cPLA2 mobility shift on SDS-PAGE

Antigone Lazou lazou at bio.auth.gr
Mon Mar 27 03:42:54 EST 2000


Hi,

I am currently working on cPLA2 activation and I am trying to detect a
mobility shift on SDS-PAGE due to phosphorylation of the protein. I have
tried several conc. of acrylamide, bis-acrylamide etc but nothing seems to
work. Does anybody have an experience on that and give me some tips?
Thanks in advance    
Dr. Antigone Lazou
Laboratory of Animal Physiology
Dept. of Zoology
School of Biology
Aristotle University of Thessaloniki
Thessaloniki 54006
Greece 

Tel:  30 31 998381
Fax:  30 31 998331
e-mail:  lazou at bio.auth.gr
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