cloning large fragments

[Jean-Christophe Billeter]

Dear Bionet-Users

Does anyone have expertise in sub-cloning big fragments (i.e.12-15kb) from
BACs into
conventional high-copy number plasmids e.g.bluescript? What kind of band
purification
method? What insert : vector ratios? additions/improvements in ligations?
What kind of
E.coli strain would you use for transformation? Furthermore, I would be
interested in
getting feedback about the biggest insert someone has managed to sub-clone
into a
plasmid.



many thx in advance
yours in appreciation

Jean-christophe

********************************
Jean-Christophe Billeter
IBLS-Division of Molecular Genetics
Anderson College, Pontecorvo building
59 Dumbarton Road
University of Glasgow
Glasgow
G11 6NU
UK

Phone: 0044-141-330-2946
email: 9902301b at student.gla.ac.uk
       jcbilleter at hotmail.com


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