cloning large fragments

Jean-Christophe Billeter 9902301b at
Mon Mar 27 05:52:18 EST 2000

Dear Bionet-Users

Does anyone have expertise in sub-cloning big fragments (i.e.12-15kb) from
BACs into
conventional high-copy number plasmids e.g.bluescript? What kind of band
method? What insert : vector ratios? additions/improvements in ligations?
What kind of
E.coli strain would you use for transformation? Furthermore, I would be
interested in
getting feedback about the biggest insert someone has managed to sub-clone
into a

many thx in advance
yours in appreciation


Jean-Christophe Billeter
IBLS-Division of Molecular Genetics
Anderson College, Pontecorvo building
59 Dumbarton Road
University of Glasgow
G11 6NU

Phone: 0044-141-330-2946
email: 9902301b at
       jcbilleter at


More information about the Methods mailing list