PCR elongation time?

Mark Denhart snoskier at terracom.net
Mon Mar 27 11:31:59 EST 2000


I never I found an elongation step necessary....I usually keep the
anneal/extend (generally between 55 and 60 C) step at 45 sec to 1 min
with great results.  I normally do a 7 min final extend at around 68C.

Mark

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