PCR/sequence through bad region

Chris LaRosa clarosa at biocomp.unl.edu
Mon Mar 27 12:13:41 EST 2000

Chris Boyd wrote:
> Chris LaRosa <clarosa at biocomp.unl.edu> wrote:
> : Betaine......is good for your problem.   You could also make a primers
> : that sits on the bad g places.   make them ggggggggggggggggggg(bases in
> : your sequence) or ggggggggggggggggggggg(N). Also CCCCCCCCCCCCCCC(bases
> : in your sequence.OR N.
Yes. It is one of the methods recommended by perkin elmer for their abi
prism kit for reading through homopolymer regions.

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