5 prime overhang removal

Peter Ashby p.r.ashby at dundee.MAPS.ac.uk
Mon Mar 27 12:17:22 EST 2000


Dear All,

I need to chew back a 5 prime overhang to produce a blunt end. According
to the Promega catalogue I could use either Mung bean nuclease or S1
nuclease. I am familiar with using the Mung bean for 3prime overhang
removal but I'm not sure about 5 prime. Can anybody comment which would be
best or recommend something else?

TIA

Peter

-- 
Peter Ashby
Wellcome Trust Biocentre
University of Dundee
Dundee, Scotland
Reverse the spam and remove to email me.




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