PCR Subcloning with restriction sites

Susanne Rohrer srohrer at immv.unizh.ch
Tue Mar 28 04:30:57 EST 2000

Nicolas Lemee wrote:

> Susanne Rohrer wrote:
> > XhoI is a bad cutter
> Not in my hands, I use it a lot, never had a problem.

Mine is, on 3bp overhangs of PCR fragments. The NEB catalogue said so,
but maybe it's the placebo effect....

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