Gene fishing

David Reeves dcr22 at cam.ac.uk
Tue Mar 28 05:43:33 EST 2000


Has anyone here ever had any luck using degenerate PCR to do cross-species
cloning? I'm unsure whether to use some sort of touchdown/step up PCR
protocol with the degerate primers or just decrease the annealing
temperature in the standard protocol. 


Thanks for your help,



David Reeves
Dept of Biochemistry
University of Cambridge, UK




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