PROs and CONs of visualization of DNA in agarose gel by EtBr

Chris LaRosa clarosa at biocomp.unl.edu
Tue Mar 28 10:37:05 EST 2000


Staining the gel and the bottom buffer with EtBr is how we do it for max
staining and sensitivity, since without adding buffer to bottom buffer,
the EtBr is eluted off the gel, reducing sensitivity.  For some reason,
gels run much better if they are pre run.




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