Sequencing by RT-PCR

Chris LaRosa clarosa at biocomp.unl.edu
Tue Mar 28 13:21:52 EST 2000


Why make life more complicated??

Simply RT-PCR your product, then sequence off the PCR product. Once you
have a nice clean band you can reamplify, subclone to a TA cloning
vector, what have you. If it is not so clean you can excise bands, or
take plugs from gels.  In our lab we routinely cycle sequence off pcr
products.
If you need more detail than that write back.

PCPhD




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