Can't freeze my comp cells!

Susanne Rohrer srohrer at
Wed Mar 29 02:52:39 EST 2000

As someone else stated, the Inoue Method is fine. What we watch out for
is to add the DMSO very slowly, i.e. dropwise while stirring the cells,
on ice, on a slow shaker. Then we just snap-freeze in LN2. Thawing them
slowly is recommended as well.


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