Can't freeze my comp cells!
hzhen at freeuk.com
Wed Mar 29 06:46:20 EST 2000
Susanne Rohrer wrote:
> As someone else stated, the Inoue Method is fine. What we watch out for
> is to add the DMSO very slowly, i.e. dropwise while stirring the cells,
> on ice, on a slow shaker. Then we just snap-freeze in LN2. Thawing them
> slowly is recommended as well.
Just a brief note: Hanahan reported that oxidised DMSO
affects the competence of cells, however, Inoue also
reported oxidation of DMSO have no effect whatsoever. So it
may behave differently for different methods?
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