Sequencing by RT-PCR

Dr. Duncan Clark Duncan at
Wed Mar 29 06:59:59 EST 2000

In article <38E1E459.222A0395 at>, Susanne Rohrer
<srohrer at> writes
>I once imagined just throwing RT into our fluorescent sequencing mix and
>seeing if it can be analyzed on the sequencer - but  it probably won't
>work, will it? (Saaay, has anyone tried it?) Wouldn't want to clog up the

RNA sequencing can be done with RT plus ddNTPs. That's how 16s rRNA's
were sequenced maybe 7-10 years ago, direct from RNA.

You will need to find a paper that gives the dNTP:ddNTP ratios. Maybe a
look for old papers by P. Lawson and/or D. Collins from the IFRC unit at
Reading, UK.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

More information about the Methods mailing list