Sequencing by RT-PCR
Dr. Duncan Clark
Duncan at nospam.demon.co.uk
Wed Mar 29 06:59:59 EST 2000
In article <38E1E459.222A0395 at immv.unizh.ch>, Susanne Rohrer
<srohrer at immv.unizh.ch> writes
>I once imagined just throwing RT into our fluorescent sequencing mix and
>seeing if it can be analyzed on the sequencer - but it probably won't
>work, will it? (Saaay, has anyone tried it?) Wouldn't want to clog up the
RNA sequencing can be done with RT plus ddNTPs. That's how 16s rRNA's
were sequenced maybe 7-10 years ago, direct from RNA.
You will need to find a paper that gives the dNTP:ddNTP ratios. Maybe a
look for old papers by P. Lawson and/or D. Collins from the IFRC unit at
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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