CAPS TaqI restriction site

Chunxin Wang cxwang at wam.umd.edu
Thu Mar 30 09:47:20 EST 2000


just a reminder the incubation temp. for Taq I is unusual 65C. also it is
blocked only by overlapping dam methylation, that is, the recognition
site is followed by TC (TCGmATC). you can check your marker sequence to
see if they are there.

Black



On Thu, 30 Mar 2000, Kwangchul Oh wrote:

> I am currently doing CAPS analysis. While I was screening various
> restriction enzymes, I've got
> weird results. There were several TaqI sites in my marker sequences that
> might show polymorphism.
> However, TaqI did not cut anything. So, I suspect the methylation on that
> site inhibited the digestion. Is there any easy way to test my assumption?
> 
> Thanks
> 
> KwangChul Oh
> 
> Oregon State University
> Botany and Plant Pathology
> Cordely Hall 2082
> Corvallis, OR 97331-2902
> 

---




More information about the Methods mailing list