"Parent, Eric" wrote: > > I'm using Boehringer Expand Long Template. I've been able to amplify a small > fragment, reverse the primers and try to amplify the mtDNA. > > Check your GC content of related organisms. In some weird slime molds, the AT richness forces you to run the pcr at 60 C with 8 min extention. How pure is your mitocondrial template.