help: long pcr problem (urgent)

Chris LaRosa clarosa at
Thu Mar 30 10:42:27 EST 2000

"Parent, Eric" wrote:
> I'm using Boehringer Expand Long Template. I've been able to amplify a small
> fragment, reverse the primers and try to amplify the mtDNA.

Check your GC content of related organisms.   In some weird slime molds,
the AT richness forces you to run the pcr at 60 C with 8 min extention.

How pure is your mitocondrial template.

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