help: long pcr problem (urgent)

Chris LaRosa clarosa at biocomp.unl.edu
Thu Mar 30 10:42:27 EST 2000



"Parent, Eric" wrote:
> 
> I'm using Boehringer Expand Long Template. I've been able to amplify a small
> fragment, reverse the primers and try to amplify the mtDNA.
> 
> 

Check your GC content of related organisms.   In some weird slime molds,
the AT richness forces you to run the pcr at 60 C with 8 min extention.

How pure is your mitocondrial template.




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