Avidin-biotin problems

[Austin P. So (Hae-Jin)]

Mark Molloy wrote:

> I am trying to purify a biotinylated peptide using a monomeric avidin
> column.
> The column is equilibrated in PBS, blocked with 2mM biotin,

Why are you doing this?! Odds are that the modified peptide has a lower
affinity for the support than free biotin so are just blocking the
association.

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Austin P. So (Hae Jin)

I.I.S.G.P.
Biotechnology Laboratory
University of British Columbia

E-mail: haejin at netinfo.ubc.ca

http://www.interchange.ubc.ca/haejin/index.html (under construction)