High molecular DNA isolation for library construction

Kwangchul Oh ohk at ava.bcc.orst.edu
Fri Mar 31 01:16:28 EST 2000

I am going to isolate high molecular weight DNA from tomato leaves to
construct library. In our lab, a former postDoc was trying to isolate
protoplast for DNA extraction but it did not work well. Thus I am
considering nuclei isolation method for DNA isolation. Moreover, referring
to several papers, it seems the nuclei isolation method is better than
protoplast isolation method.
Does anybody have suggestion before I start this?

KwangChul Oh

Oregon State University
Botany and Plant Pathology
Cordely Hall 2082
Corvallis, OR 97331-2902

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