PROs and CONs of visualization of DNA in agarose gel by EtBr

Chris LaRosa clarosa at biocomp.unl.edu
Fri Mar 31 10:04:38 EST 2000


  One
> thing to note is that EtBr runs in the opposite direction from nucleic
> acids so if you add to the gel or the sample you will see some EtBr
> artifacts in the gel, it might be brighter in half of the gel.  I have
> not experienced any difficulty in visualizing gels because of this and
> since EtBr intercollates with nucleic acids, it doesn't seem to come out
> of the sample once it's intercollated even though it runs off the gel.
> 
>
As I said before , I add 0.025 percent ETBR to the bottom buffer, if we
do 
not we do notice that a loss of sensitivity, apparently intercollating
is irreversable or some other interaction stains also.




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