Pierce T-Gel kit??
rew1000 at nospam.cam.ac.uk
Tue May 2 11:59:11 EST 2000
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> Hi there,
> I want to use the Pierce T-gel kit for IgG purification.
> So, anybody out there who knows the buffer composiions and protocols or
> who knows what (published) method they follow??
> Any input apreciated, Thanks Ina
All the product manuals are at www.piercenet.com
This one is at
The buffers are
T-Gel T Binding Buffer, 1000 ml ,0.5 M potassium sulfate, 50 mM sodium
0.05% sodium azide, pH 8.0.
T-Gel T Elution Buffer, 1000 ml, 50 mM sodium phosphate, 0.05% sodium azide,
2X Concentrated T-Gel T Column Storage Buffer, 100 ml 1 M Tris and 0.05%
sodium azide, pH 7.4.
Institute of Biotechnology, University of Cambridge
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