Some Minor Questions Re SDS PAGE :
mehdia at interchange.ubc.ca
Sun May 7 15:38:35 EST 2000
I need answers to the following questions:
1. Should the protein standards be diluted with loading buffer before
it is not needed?
2. Do the protein standards also need to be boiled before loading?
3. Once boiled for 5 min with loading buffer, the protein stock samples
stored in -20 but do they need to be boiled again each time before
4. When I have far different concentrations of proteins, do I need to
precipitate and dilute again in order to have approximately similar
concentrations within equal total volume, in order to have a perfect run?
Thanks, in advance
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