Some Minor Questions Re SDS PAGE :

Arnoud van Vliet avvliet at knoware.nl
Mon May 8 16:01:03 EST 2000


Once you have boiled them, and stored them at -20 degrees C, there is
absolutely no need to reboil or even heat them to 65 degrees. Just thawing
them will do. And the students here learn to do that behind their ears; just
the place where you'd like some B-mercaptoethanol :)

Arnoud

>
> > 3.  Once boiled for 5 min with loading buffer, the protein stock samples
> > could be stored in -20 but do they need to be boiled again each time
before loading?
> Yes.







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