Some Minor Questions Re SDS PAGE :

Susanne Rohrer "srohrer(removethis)" at
Tue May 9 02:48:03 EST 2000

> > 3.  Once boiled for 5 min with loading buffer, the protein stock samples
> > could be stored in -20 but do they need to be boiled again each time before loading?
> > Yes.

I don't do it usually. with time/repeated boiling the proteins will degrade.

Susanne Rohrer
Institute of Medical Microbiology
University of Zurich

srohrer at immv dot unizh dot ch

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