Some Minor Questions Re SDS PAGE :

[Susanne Rohrer]

>
>
> > 3.  Once boiled for 5 min with loading buffer, the protein stock samples
> > could be stored in -20 but do they need to be boiled again each time before loading?
> > Yes.

I don't do it usually. with time/repeated boiling the proteins will degrade.


--
Susanne Rohrer
Institute of Medical Microbiology
University of Zurich
Switzerland

srohrer at immv dot unizh dot ch