cosmid/genomic DNA ligation

Hiranya S. Roychowdhury hroychow at nmsu.edu
Tue May 9 11:53:29 EST 2000


At 08:25 AM 5/9/00 -0500, Rodney Earl Pettway wrote:
>I am going to be trying to make a cosmid library.  My cosmid has two cos
>sites. 

no kidding!?

 I was wondering if anyone could recommend a protocol for the
>ligation reaction eg. concentrations of cosmid and genomic DNA in the
>ligation reaction.

check out Maniatis. Great protocol available there.


>
>thank you very much and have a great day!!
>
>


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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