High temp. restriction enzyme digests

Hiranya S. Roychowdhury hroychow at nmsu.edu
Tue May 9 13:10:43 EST 2000


Good, sterilized, mineral oil is something that I have used for BclI, BstXI etc.

Another trick is to incubate the tube up-side-down (ie. on its cap) in a 50
C incubator oven.




At 12:30 PM 5/9/00 -0400, Kunal Mukhopadhyay wrote:
>I am trying to digest a plasmid with BclI whose optimum temperature is at
>50C. With time, water evaporates and obviously affects the reaction
>environment. I am aware of some simple tricks like using mineral oil, but am
>not certain. Anyone with some experience - if could kindly give some
>pointers would be HIGHLY appreciated!!
>
>Thanks
>Kunal
>
>--
>
>
>****************************************************************************
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>What does it profit a man if he gains the world... but loses his soul?
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>Kunal Mukhopadhyay
>
>52, 152 Davey Lab,
>Department Of Chemistry
>Pennsylavania State University,
>State College, PA 16802
>USA
>
>(814) 865-1273
>
>email : kxm66 at psu.edu.
>
>
>
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
>>>
>
>
>


Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu

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