High temp. restriction enzyme digests

Chris LaRosa clarosa at biocomp.unl.edu
Tue May 9 16:46:43 EST 2000



"Michael L. Sullivan" wrote:
> 
> >I am trying to digest a plasmid with BclI whose optimum temperature is at
> >50C. With time, water evaporates and obviously affects the reaction
> >environment. I am aware of some simple tricks like using mineral oil, bu



also can use thermocycler with heated lids....

PCPhD.




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