High temp. restriction enzyme digests

Susanne Rohrer "srohrer(removethis)" at immv.unizh.ch
Wed May 10 02:09:55 EST 2000


Do it submerged in a waterbath (seal the tube w/ Parafilm) or do it in a 50°C
Incubator.

Kunal Mukhopadhyay wrote:

> I am trying to digest a plasmid with BclI whose optimum temperature is at
> 50C. With time, water evaporates and obviously affects the reaction
> environment. I am aware of some simple tricks like using mineral oil, but am
> not certain. Anyone with some experience - if could kindly give some
> pointers would be HIGHLY appreciated!!
>
> Thanks
> Kunal
>
> --
>
> ****************************************************************************
> ******
> What does it profit a man if he gains the world... but loses his soul?
> ****************************************************************************
> ******
>
> Kunal Mukhopadhyay
>
> 52, 152 Davey Lab,
> Department Of Chemistry
> Pennsylavania State University,
> State College, PA 16802
> USA
>
> (814) 865-1273
>
> email : kxm66 at psu.edu.
>
> >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
> >>

--
Susanne Rohrer
Institute of Medical Microbiology
University of Zurich
Switzerland

srohrer at immv dot unizh dot ch






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