GFP western blot from Drosophila embryos

Hansi hase at nowhere.com
Fri May 12 03:52:01 EST 2000


We use the monoclonal abs mix from Boehringer Mannheim (=Roche these
days...) and
never had a problem. I know that the abs that Clontech sells do not work
(at least in our hands)

Daniel

Sven Pfeiffer wrote:

> Hi there,
>
>         has anyone done Western blots with a GFP antibody from embryos?
> My
> own efforts are rather poor, lots of unspecific bands it seems. I am
> looking for a clean antibody and a reliable protocol.
>
> Cheers
>
> Sven
>
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>
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