Internal deletions by PCR
Wolfgang.Schechinger at med.uni-tuebingen.de
Fri May 12 05:33:05 EST 2000
you could try the quick change protocol with a complementary primer
pair in order to "loop out" the unwanted region.
Yust an idea, has anyone tried this yet?
> From: "Ulf S. Andersson" <u_andersson2 at yahoo.com>
> Subject: Internal deletions by PCR
> Date: Thu, 11 May 2000 16:02:19 -0500
> Organization: Northwestern University, Evanston, IL, US
> To: methods at hgmp.mrc.ac.uk
> I wonder if anybody could tip me on a good and fast way of making
> internal deletions in a plasmid by PCR. I have previously done it by
> introducing new restriction sites by site directed mutagenesis (and
> then digesting and re-ligating) which takes quite a long time...
> there must be a smarter way. /Ulf.
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de
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