ligation reaction problems!!

Rodney Earl Pettway earlpettway at netscape.net
Fri May 12 15:55:05 EST 2000


I would like a detailed protocol for the ligation of cosmid DNA with
genomic DNA.
I have one but it ask me to set up a reaction containing a 9:1 molar
ratio of  vector DNA:eukaryotic DNA.  I don't know how to figure out the
molar concentrations.

thanks

Rodney Earl Pettway
USDA-ARS
Cereal Disease Lab
University of Minnesota







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