DNA polymerase and RNA template

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Sun May 14 15:00:05 EST 2000


In article <391EDDA1.F0FF3F43 at mail.ls.huji.ac.il>,
yoramg2 at cezanne.ls.huji.ac.il (Yoram Gerchman) wrote:
>Greetings Netters
>Just wondering, if I have a plasmid with a stretch of RNA in
the middle.
>My question is: will this plasmid replicate as usual and will
the RNA be
>replaced with DNA?
>--

It should, in a normal bacteria anyway. It will probably be seen
as an Okazaki fragment, and be replaced by DNA (by Pol I, IIRC,
but my memroy could be faulty on this point.)

Nick

Nick Theodorakis

nicholas_theodorakis at urmc.rochester.edu
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