b-gal assays from LB cultures?

Don Walthers gene_jockey26 at yahoo.com
Sun May 14 19:04:20 EST 2000

Are there any drawbacks to performing b-gal assays as described in Maniatis
from LB cultures? The lacZ is fused to the lexA promoter so catabolite
repression is not an issue. I need to select for 4 different antibiotics and
the cultures do not grow up well in A medium with all the drugs. Will LB be
compatible with Z buffer or should I give the cells a wash.
Thanks for any info,

Don Walthers
BS Microbiology, U of Illinois at UC, 1996
PhD Microbiology, U of Idaho, 2000?
gene_jockey26 at uidaho.edu

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