Anaerobic expression of GFP?

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Mon May 15 03:55:23 EST 2000


Hi all out there, here is this weeks's problem:

I'd like to express and His-tag purify GFP expressed preferably in 
E. coli. But I need to prevent the formation of the chromophore wich 
is formed by oxidation.
Any ideas?

All input is welcome

Wolfgang
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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