Anaerobic expression of GFP?

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Mon May 15 07:24:14 EST 2000


"Wolfgang Schechinger" <Wolfgang.Schechinger at med.uni-tuebingen.de> wrote:
> I'd like to express and His-tag purify GFP expressed preferably in 
> E. coli. But I need to prevent the formation of the chromophore wich 
> is formed by oxidation.
> Any ideas?

Mutation of one of the three amino acids that form the chromophore?
This should yield a non-fluorescent protein that most likely folds in
the same way than GFP.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */




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