Anaerobic expression of GFP?
krasel at wpxx02.toxi.uni-wuerzburg.de
Mon May 15 07:24:14 EST 2000
"Wolfgang Schechinger" <Wolfgang.Schechinger at med.uni-tuebingen.de> wrote:
> I'd like to express and His-tag purify GFP expressed preferably in
> E. coli. But I need to prevent the formation of the chromophore wich
> is formed by oxidation.
> Any ideas?
Mutation of one of the three amino acids that form the chromophore?
This should yield a non-fluorescent protein that most likely folds in
the same way than GFP.
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
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