blue/white selection problem

Gary gkrause at med.wayne.edu.spam
Mon May 15 14:26:13 EST 2000


"Rodney Earl Pettway" <earlpettway at netscape.net> wrote in message
news:3920455F.1B331B94 at netscape.net...
> I am using blue/white selection to choose colonies with my insert.
> Unfortunately, my white colonies don't have inserts.  What could be
> causing this.
>
> Rodney Earl Pettway
> USDA-ARS Cereal Disease Lab
> University of Minnesota
> St. Paul, MN., 55108
>


You would get this is your insert is a multiple of three so that there is no
reading frame shift.  This would also require that there be no stop codons
in the reading frame.

Gary








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