blue/white selection problem

Rodney Earl Pettway earlpettway at netscape.net
Mon May 15 15:05:35 EST 2000


Well, I asked the person who is having this problem and she says that
the vector comes in a kit and it is suppose to be dephosphorylated
already.  Well?????

"Hiranya S. Roychowdhury" wrote:

> Concatenation of the vector is a distinct possibility. Are you using
> dephosphorylated vector? If not, do it... you'll see the difference!
>
> At 01:43 PM 5/15/00 -0500, Rodney Earl Pettway wrote:
> >I am using blue/white selection to choose colonies with my insert.
> >Unfortunately, my white colonies don't have inserts.  What could be
> >causing this.
> >
> >Rodney Earl Pettway
> >USDA-ARS Cereal Disease Lab
> >University of Minnesota
> >St. Paul, MN., 55108
> >
> >
>
> Dr. Hiranya Sankar Roychowdhury
> GENE LAB/ EPPWS
> New Mexico State University
> Las Cruces, NM 88003
> Ph. (505) 646-5785
> hroychow at nmsu.edu
>
> ---





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