Anaerobic expression of GFP?
Wolfgang.Schechinger at med.uni-tuebingen.de
Tue May 16 03:20:03 EST 2000
Your proposal is a very good idea for studying the correct folding. I
still want to render my GFP fluorescent, but *after* the expresseion,
somewhat controlled. The chemistry of the chromophor is interfering
with my experiment since it doesn't consist of amino acids.
> "Wolfgang Schechinger" <Wolfgang.Schechinger at med.uni-tuebingen.de>
> wrote: > I'd like to express and His-tag purify GFP expressed
> preferably in > E. coli. But I need to prevent the formation of the
> chromophore wich > is formed by oxidation. > Any ideas?
> Mutation of one of the three amino acids that form the chromophore?
> This should yield a non-fluorescent protein that most likely folds
> in the same way than GFP.
> /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher
> Str. 9 */ /* D-97078 Wuerzburg, Germany email:
> phak004 at rzbox.uni-wuerzburg.de SP4 */ /* "Science is the game we
> play with God to find out what His rules are." */
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Dr. Wolfgang Schechinger, Dept. of Pathobiochemistry
University of Tuebingen, Germany
email: wolfgang.schechinger at med.uni-tuebingen.de
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