Richard P. Grant
rgrant at netscape.net
Tue May 16 04:50:26 EST 2000
In article <39210B81.C499013E at cib.csic.es>, P.Hernandez at cib.csic.es
> When using isopropanol for DNA precipitation, is it necessary to add any
> kind of salt before?
No, there is no need to add salt. Be careful not to leave the ppt too
long at low temp as you can get a lot of protein coming down. What is
the particular application here?
> I have read that after isopropanol precipitation it
> is a good idea to wash the pellet to remove coprecipitated staff. This
> washing should be with 70% isopropanol, ethanol or what ?
70% Ethanol is a good idea, but if you want to be rigorous you should
resuspend the pellet and reprecipitate with acetate/ethanol.
Richard P. Grant MAD Phil http://www.gerbil.org.uk/
Please reply to rpg 'at' mrc-lmb.cam.ac.uk'
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