Isopropanol

[Richard P. Grant]

In article <39210B81.C499013E at cib.csic.es>, P.Hernandez at cib.csic.es 
wrote:

> Hi,
> 
> When using isopropanol for DNA precipitation, is it necessary to add any
> kind of salt before? 

No, there is no need to add salt.  Be careful not to leave the ppt too 
long at low temp as you can get a lot of protein coming down.  What is 
the particular application here?

> I have read that after isopropanol precipitation it
> is a good idea to wash the pellet to remove coprecipitated staff. This
> washing should be with 70% isopropanol, ethanol or what ?

70% Ethanol is a good idea, but if you want to be rigorous you should 
resuspend the pellet and reprecipitate with acetate/ethanol.

R

-- 
Richard P. Grant MAD Phil        http://www.gerbil.org.uk/    
Please reply to rpg 'at' mrc-lmb.cam.ac.uk'

         Sex and bugs and rock and roll