Adding restriction sites to PCR primers

Beverly Metcalfe beverly829 at hotmail.com
Tue May 16 08:40:18 EST 2000


I am designing primers and I want to add restriction enzyme sites to the 
5'ends.  To do this, do I just add the restriction site to the 5' end, or do 
I need to add extra bases after the enzyme's site so the the enzyme will be 
able to cleave it?
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