blue/white selection problem

Rodney Earl Pettway earlpettway at netscape.net
Tue May 16 09:48:33 EST 2000


Thanks!! I am not sure if she has done that control yet.  I will find out.

have a great day.

"Hiranya S. Roychowdhury" wrote:

> Well???? Indeed.
> The problem you had described pointed in that general direction. It is also
> possible that if the vector in the kit is supposed to come dephosphorylated,
> there was a QC glitch. After all, these are processed by humans.
> Has she done any ligation w/o the insert? This has to be one control that
> should never be dispensed with... not even for those vectors that are known
> to work.
>
> At 03:05 PM 5/15/00 -0500, Rodney Earl Pettway wrote:
> >Well, I asked the person who is having this problem and she says that
> >the vector comes in a kit and it is suppose to be dephosphorylated
> >already.  Well?????
> >
> >"Hiranya S. Roychowdhury" wrote:
> >
> >> Concatenation of the vector is a distinct possibility. Are you using
> >> dephosphorylated vector? If not, do it... you'll see the difference!
> >>
> >> At 01:43 PM 5/15/00 -0500, Rodney Earl Pettway wrote:
> >> >I am using blue/white selection to choose colonies with my insert.
> >> >Unfortunately, my white colonies don't have inserts.  What could be
> >> >causing this.
> >> >
> >> >Rodney Earl Pettway
> >> >USDA-ARS Cereal Disease Lab
> >> >University of Minnesota
> >> >St. Paul, MN., 55108
> >> >
> >> >
> >>
> >> Dr. Hiranya Sankar Roychowdhury
> >> GENE LAB/ EPPWS
> >> New Mexico State University
> >> Las Cruces, NM 88003
> >> Ph. (505) 646-5785
> >> hroychow at nmsu.edu
> >>
> >> ---
> >
> >
>
> Dr. Hiranya Sankar Roychowdhury
> GENE LAB/ EPPWS
> New Mexico State University
> Las Cruces, NM 88003
> Ph. (505) 646-5785
> hroychow at nmsu.edu
>
> ---





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