Isopropanol

[Sergio]

Pablo Hernandez wrote:

> Hi,
>
> When using isopropanol for DNA precipitation, is it necessary to add any
> kind of salt before?

Yes, usually AcNa or AcK 0,3 M, unless the sample already contains another
kind of salts (like the miniprep sol III).

> I have read that after isopropanol precipitation it
> is a good idea to wash the pellet to remove coprecipitated staff. This
> washing should be with 70% isopropanol, ethanol or what ?

Ethanol. This removes the coprecipitated salts and the isopropanol.


Espero haberte ayudado.
PS: un monton de gente del CIB realiza minipreps cotidianamente. Pregunta
por alli y te explicaran cualquier duda.

Sergio