RANDOM primer labelling efficiency

Mano mdep at musica.mcgill.ca
Tue May 16 22:46:18 EST 2000


Hi all,
my collegue does not denature the DNA template before random primer
labelling and claims to get efficient incorporation of 32P-dCTP.  Is
denaturation of the template a "MUST"?  I always do , nevertheless.

Mano





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