RANDOM primer labelling efficiency

Pablo Hernandez P.Hernandez at cib.csic.es
Wed May 17 06:50:25 EST 2000


In my experience, you have to denature the DNA for a good labeling. This
makes sense, since primers must  aneal to the DNA in order to be
extended.



Mano wrote:

> Hi all,
> my collegue does not denature the DNA template before random primer
> labelling and claims to get efficient incorporation of 32P-dCTP.  Is
> denaturation of the template a "MUST"?  I always do , nevertheless.
>
> Mano
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