RANDOM primer labelling efficiency
P.Hernandez at cib.csic.es
Wed May 17 06:50:25 EST 2000
In my experience, you have to denature the DNA for a good labeling. This
makes sense, since primers must aneal to the DNA in order to be
> Hi all,
> my collegue does not denature the DNA template before random primer
> labelling and claims to get efficient incorporation of 32P-dCTP. Is
> denaturation of the template a "MUST"? I always do , nevertheless.
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the Methods