RANDOM primer labelling efficiency

Chris LaRosa clarosa at biocomp.unl.edu
Wed May 17 08:21:59 EST 2000


> my collegue does not denature the DNA template before random primer
> labelling and claims to get efficient incorporation of 32P-dCTP.  Is
> denaturation of the template a "MUST"?  I always do , nevertheless.
> 
> Mano
If you fail to denature you will always fail to get efficient
incorporation.....How can the primers bind to sites if they are already
taken??




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