RANDOM primer labelling efficiency

George Rutherford gruther at bilbo.bio.purdue.edu
Wed May 17 14:27:16 EST 2000


In article <39221670.C3DC6CE0 at musica.mcgill.ca>, Mano
<mdep at musica.mcgill.ca> wrote:

> Hi all,
> my collegue does not denature the DNA template before random primer
> labelling and claims to get efficient incorporation of 32P-dCTP.  Is
> denaturation of the template a "MUST"?  I always do , nevertheless.
> 


I once knew a rookie grad student who couldn't get his RP rxns to work. I
asked him if he was denaturing his template. He gave me a blank look which
I took for a "no". His rxns started working.

George




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