2d gel cleanup

Andrea Beckel-Mitchener amitch at uiuc.edu
Fri May 19 16:20:52 EST 2000


I'm not sure what you mean by "fixing".  I dehydrate with 50%
Methanol/2%glycerol for 10-15 min before drying.  If the background is due to
unincorporated label, this treatment doesn't get rid of it.
A.

>
>
> Are you fixing the gels beore drying them? Usually, fixation and
> washing should wash out the unincorporated label.
>

--
Andrea Beckel-Mitchener, PhD
Beckman Institute
University of Illinois






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