Desalting with butanol precipitation (w/ a reminder)

Jouko Kalevi Pettersson petterss at cc.helsinki.fi
Fri May 19 18:56:51 EST 2000


In article <200005191948.NAA260066 at nestor.NMSU.Edu>, Hiranya S. Roychowdhury
wrote:

>BTW, butanol is immiscible with water (although it absorbs it) and will NOT
>precipitate DNA or RNA as you originally enquired.

It absorbs about 10% water. It is used to precipitate oligonucleotides.

http://grimwade.biochem.unimelb.edu.au/bowtell/molbiol/sect23.htm
-- 
Jouko Pettersson              Internet: Jouko.Pettersson at Helsinki.FI
Division of Biochemistry      FAX:      +358 9 191 59068
Department of Biosciences     Phone:    +358 9 191 59012
University of Helsinki        http://www.helsinki.fi/~petterss/





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