2d gel cleanup

Chris LaRosa clarosa at biocomp.unl.edu
Sat May 20 11:01:28 EST 2000


> 
> As for precipitation, I agree that TCA might be a dodgy choice
> before IEF, but perhaps acetone pptn, would be ok.
> 
In the old days, For preparation of 2 d proteins, we would typically do
a methanol/chloroform precipitation and concentration step.  Might this
remove unincorporated amino acids?




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