SDS-PAGE for low nolecular weight peotide

rogier stugerNOstSPAM at cellbiology.uni-frankfurt.de.invalid
Mon May 22 13:00:36 EST 2000


MW 1600, difference 14... that's less than 1 %. mw 14 is less
than an amino acid! It's the nitrogen in a peptide bond ...
SDS-PAGE will not work here, not even with a tricine-gel
(Schagger/vonJagow system).
You better try a mass spec. This will easily tell the difference.
Cya,
Rogier Stuger
Mol Cel Physiol, Free U of Amsterdam
E rogier AT bio.vu.nl
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