Clone DNA fragments from dried PAGE gel

kkelnar at my-deja.com kkelnar at my-deja.com
Wed May 24 10:41:48 EST 2000


we do this all the time...
cut the gel and put it in a 1.5ml microcentrifuge tube.  add ~60 ul
water and place at 95 degrees for ten minutes.  we have successfully
done pcr from this solution.
kevin kelnar

In article <3.0.32.20000522110427.008ff2d0 at weizen.ethz.ch>,
  zhiyong.liu at ipw.agrl.ethz.ch (Zhiyong Liu) wrote:
> Dear friends,
>
> I would like to clone some DNA fragments from dried PAGE gel (two
years old). Is anyone could give me some practical protocol?
>
> Thanks a lot
>
> Liu
>
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