SDS-PAGE for low nolecular weight peotide

Frank O. Fackelmayer Frank.Fackelmayer at uni-konstanz.de
Thu May 25 04:06:31 EST 2000


"Li, Jian - LIYJY005" wrote:
> 
> Hi, All,
> 
> Is it possible to use SDS-PAGE to seperate two peptides (Mw 1600 or so) with
> molecular difference of 14? Many thanks!
> 
>

definitely not. The only thing that will work here is mass spectrometry,
e.g. MALDI-TOF. By the way: where does the possible difference of 14
come from? It is way smaller than one amino acid, so it must be some
side chain modification, is it methylation? 

Frank






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