DNA supercoiling

Dr. R O'Kennedy r.o'kennedy at ucl.ac.uk
Thu May 25 10:39:15 EST 2000

I'm interesting in assaying for alterations in plasmid DNA supercoiled 
linking number using chloroquine gel electrophoresis. Most of the 
papers use small plasmid DNA such as pBR322 as a "supercoiling probe" 
and I would like to be able to go up to 20 Kb. Does anybody know what 
the size limitations are of this technique  ?



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